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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 2, 1998, pp. 237-241
Bioline Code: oc98045
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 2, 1998, pp. 237-241

 en Properties of beta-lactamase from Neisseria gonorrhoeae
Marta C de Castillo; Fernando Sesma; Olga M de Nader & Aida P de Ruiz Holgado

Abstract

Beta-lactamase activity was studied in Neisseria gonorrhoeae
strains. Optimum temperature was found to be 37 C. The enzyme was
inactivated at temperatures higher than 60 C, but remained active during
storage at low temperatures (4 C, -30 C and -70 C) for two months. Enzyme
activity was observed within a pH range of 5.8-8.0, while the optimum pH
was 7.0-7.2. Addition of Ni^2+, Fe^2+, Fe^3+, Mn^2+ and
p-chloromercurybenzoate to the reaction buffer exerted a negative effect
upon the activity, whereas Hg^2+ and ethylene diamine tetra-acetic acid
produced complete inhibition. These results would indicate the presence of
-SH groups at the catalytic site of the enzyme.

Keywords
Neisseria gonorrhoeae - beta-lactamase - enzyme activity

 
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