Thirty-four Leishmania isolates obtained from Costa Rican
patients, from different geographical areas, were characterized by
isoenzyme electrophoresis and indirect immunofluorescense with monoclonal
antibodies. Thirty-two were characterized as L. panamensis strains
and two were L. braziliensis variants. We confirm the evident
predominance of L. panamensis as the main etiological agent of
leishmaniasis in Costa Rica and the existence of L. braziliensis in
the country.