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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 4, 1998, pp. 485-486
Bioline Code: oc98094
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 4, 1998, pp. 485-486

 en RESEARCH NOTE - Random by amplified polymorphic DNA analysis of sylvatic Trypanosoma cruzi isolates infers from French Guiana accurate phylogeny
Brigitte Bastrenta & Simone Frederique Breniere

Abstract

Trypanosoma cruzi, the agent of Chagas disease, exhibits considerable
genetic diversity as evidenced by multilocus enzyme electrophoresis (MLEE)
and presents a basically clonal structure. Clonality in T. cruzi has
been mainly explored in domestic cycles. Nevertheless, in sylvatic cycles
the possibility of genetic exchange could be more frequent. However, recent
MLEE analysis of wild T. cruzi stocks from French Guiana suggested
that these sylvatic populations are basically clonal too. Moreover, a
previous study of several species of parasites showed that random amplified
polymorphic DNA (RAPD) was a suitable tool for evolutionary studies in
pathogenic microorganisms.

Keywords
Trypanosoma cruzi - sylvatic cycle - RAPD - synapomorphic character

 
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