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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 4, 1998, pp. 487-490
Bioline Code: oc98095
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 4, 1998, pp. 487-490

 en Evaluation of three Mycobacterium leprae monoclonal antibodies in mucus and lymph samples from Ziehl-Neelsen stain negative leprosy patients and their household contacts in an Indian community
Nora Cardona-Castro; Gustavo Ortega-Rodriguez & Piedad Agudelo-Florez

Abstract

Mucus and lymph smears collected from leprosy patients (9) and their
household contacts (44) in the Cano Mochuelo Indian Reservation, Casanare,
Colombia, were examined with monoclonal antibodies (MoAb) against
Mycobacterium leprae. The individuals studied were: 5 borderline
leprosy (BB) patients, 4 with a lepromatous leprosy (LL), all of whom were
undergoing epidemiological surveillance after treatment and 44 household
contacts: 21 of the LL and 23 contacts of the BB patients. 

The MoAb were reactive with the following M. leprae antigens: 65 kd heat shock protein, A6; soluble antigen G7 and complete antigen, E11. All the samples were tested with each of the MoAb using the avidin-biotin-peroxidase technique and 3,3 diaminobenzidine as chromogen. The patients and household contacts studied were all recorded as Ziehl-Neelsen stain negative.

The MoAb which showed optimal reaction was G7, this MoAb permited good visualization of the bacilli. Five patients with BB diagnosis and one with LL were positive for G7; of the BB patients' household contacts, 9 were positive for G7; 7 of the LL patients' household contacts were positive for the same MoAb. MoAb G7 allowed the detection of bacillar Mycobacterium spp. compatible structures in both patients and household contacts. G7 permited the visualization of the complete bacillus and could be used for early diagnosis and follow-up of the disease in patients.

Keywords
monoclonal antibodies - Mycobacterium leprae - lymph and mucus samples

 
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