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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 4, 1998, pp. 509-514
Bioline Code: oc98099
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 4, 1998, pp. 509-514

 en Morphometric analysis of McCoy cells inoculated with cerebrospinal fluid from patients with Rabies
Yeda L Nogueira

Abstract

To demonstrate the potential of McCoy cells for the isolation of rabies
virus from the cerebrospinal (CSF) fluid of a patient with a diagnosis of
rabies, McCoy cells were inoculated with CSF from a patient with a clinical
diagnosis of rabies and investigated in terms of morphometric aspect using
the JAVA analysis system for the quantification of the increased size of
infected cells compared to noninfected cells. The cells were also examined
in terms of specific staining for the diagnosis of rabies by the method of
Sellers for the observation of intracytoplasmic inclusions and by specific
immunofluorescence staining for rabies virus. 

Infected cells showed changes in cell permeability and morphologic modifications which differed significantly compared to normal cells (P{0.001) when analyzed by the Mann-Whitney and Kruskal-Wallis tests. Intense activity of the endoplasmic reticulum was also observed, as indicated by the presence of intracytoplasmic inclusions visualized by specific staining.

The present study demonstrated the isolation of rabies virus from the CSF of a patient with rabies, showing that McCoy cells can be used for the laboratory diagnosis of patients suspected to have rabies.

Keywords
image analysis - McCoy cell line - rabies virus - cerebrospinal fluid - morphometry - patient with rabies diagnosis

 
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