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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 5, 1998, pp. 589-594
Bioline Code: oc98114
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 5, 1998, pp. 589-594

 en Molecular Genetic Analysis of Multi-drug Resistance in Indian Isolates of Mycobacterium tuberculosis
Noman Siddiqi; Md. Shamim; NK Jain; Ashok Rattan; Amol Amin; VM Katoch; SK Sharma; Seyed & Hasnain

Abstract

A total of 116 isolates from patients attending the
out-patient department at the All India Institute of Medical
Sciences, New Delhi and the New Delhi Tuberculosis Centre, New
Delhi, India were collected. They were analyzed for resistance to
drugs prescribed in the treatment for tuberculosis. The drug
resistance was initially determined by microbiological
techniques. The Bactec 460TB system was employed to determine the
type and level of resistance in each isolate. The isolates were
further characterized at molecular level. The multi-drug loci
corresponding to rpo β, gyr A, kat
G were studied for mutation(s) by the polymerase chain
reaction-single strand conformational polymorphism (PCR-SSCP)
technique. The SSCP positive samples were sequenced to
characterize the mutations in rpo β, and gyr A loci. While
previously reported mutations in the gyr A and rpo
β loci were found to be present,
several novel mutations were also scored in the rpo 
β locus. 

Interestingly, analysis of the gyr A locus showed the presence of point mutation(s) that could not be detected by PCR-SSCP. Furthermore, rifampicin resistance was found to be an important marker for checking multi-drug resistance (MDR) in clinical isolates of Mycobacterium tuberculosis. This is the first report on molecular genetic analysis of MDR tuberculosis one from India, highlights the increasing incidence of MDR in the Indian isolates of M. tuberculosis.

Keywords
clinical isolates - gyr A gene - multi-drug resistance - Mycobacterium tuberculosis - rpo b gene - PCR-SSCP

 
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