About Bioline  All Journals  Testimonials  Membership  News  Donations

Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 5, 1998, pp. 601-607
Bioline Code: oc98116
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 5, 1998, pp. 601-607

 en The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes
MAS Baptista; JRC Andrade; ACP Vicente; CA Salles & A Coelho


The pathogenic O1 Amazonia variant of Vibrio cholerae
has been shown previously to have a cytotoxin acting on
cultured Vero and Y-1 cells, and to lack important virulence
factors such as the cholera toxin (Coelho et al. 1995a). This
study extends the molecular analysis of the Amazonia strains,
detecting the presence of the toxR gene, with a very
similar sequence to that of the El Tor and classical biotypes.
The outer membrane proteins are analyzed, detecting a variation
among the group of Amazonia strains, with three different
patterns found. As a by-product of this work a polymerase chain
reaction fragment was sequenced, reading part of the sequence of
the Lon protease of the Amazonia strains. This gene was not
previously described in V. cholerae, but its sequence is
present in the TIGR database specific for this species. 

Vibrio cholerae - Amazonia - toxR - outer membrane proteins - protease - Lon

© Copyright 1998 Fundacao Oswaldo Cruz - Fiocruz
Alternative site location:

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2018, Site last up-dated on 17-Sep-2018.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil