Trypanosoma cruzi, the causative agent of Chagas' disease is a highly pleomorphic parasite with a complex life cycle involving both a vertebrate host and an invertebrate vector. Several studies have been done because of its large biological and genetic variability. One of the methods that has been widely used for characterization of T. cruzi strains is isoenzyme analysis. P Ready and M Miles defined three zymodemes termed I (Z1), II (Z2) and III (Z3), based on Brazilian strains.
