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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 94, No. 1, 1999, pp. 23-29
Bioline Code: oc99009
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 1, 1999, pp. 23-29

 en Comparative Analysis by Polymerase Chain Reaction Amplified Minicircles of Kinetoplast DNA of a Stable Strain of Trypanosoma cruzi from São Felipe, Bahia, its Clones and Subclones: Possibility of Predominance of a Principal Clone in this Area
Rozalia F Campos; Marilda S Gonçalves; Eliana AG dos Reis; Mitermayer G dos Reis & Sonia G Andrade

Abstract

Molecular characterization of one stable strain of Trypanosoma cruzi, the 21
SF, representative of the pattern of strains isolated from the endemic area of São
Felipe, State of Bahia, Brazil, maintained for 15 years in laboratory by serial passages in mice
and classified as biodeme Type II and zymodeme 2 has been investigated. The kinetoplast
DNA (kDNA) of parental strain, 5 clones and 14 subclones were analyzed. Schizodeme was
established by comparative study of the fragments obtained from digestion of the 330-bp
fragments amplified by polymerase chain reaction (PCR) from the variable regions of the
minicicles, and digested by restriction endonucleases Rsa I and Hinf I. Our results show a high
percentual of similarity between the restriction fragment lenght polymorphism (RFLP) for the
parental strain and its clones and among these individual clones and their subclones at a level
of 80 to 100%.This homology indicates a predominance of the same "principal clone" in the
21SF strain and confirms the homogeneity previously observed at biological and isozymic
analysis. These results suggest the possibility that the T. cruzi strains with similar
biological and isoenzymic patterns, circulating in this endemic area, are representative of one
dominant clone. 

The presence of "principal clones" could be responsible for a predominant tropism of the parasites for specific organs and tissues and this could contribute to the pattern of clinico-pathological manifestations of Chagas's disease in one geographical area.

Keywords
Trypanosoma cruzi - strains - clones - molecular characterization - schizodeme - Bahia - Brazil

 
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