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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 94, No. 1, 1999, pp. 95-100
Bioline Code: oc99019
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 1, 1999, pp. 95-100

 en Increase of a Calcium Independent Transglutaminase Activity in the Erythrocyte during the Infection with Plasmodium falciparum
Moisés Wasserman; Ana Mercedes Márquez; Mauricio Urquiza & Patricia Jiménez


We have studied the activity of a calcium dependent transglutaminase (EC
during the growth of the parasite Plasmodium falciparum inside the infected human
erythrocyte. There is only one detectable transglutaminase in the two-cell-system, and its
origin is erythrocytic. No activity was detected in preparations of the parasite devoid of
erythrocyte cytoplasm. The Michaelis Menten constants (Km) of the enzyme for the substrates
N'N'dimethylcaseine and putrescine were undistinguishable whether the cell extracts used in
their determination were obtained from normal or from infected red cells. The total activity of
transglutaminase in stringently synchronized cultures, measured at 0.5mM
Ca2+, decreased with the maturation of the parasite. However, a fraction
which became irreversibly activated and independent of calcium concentration was detected.
The proportion of this fraction grew with maturation; it represented only 20% of the activity in
20 hr-old-trophozoites while in 48-hr-schizonts it was more than 85% of the total activity. The
activation of this fraction of transglutaminase did not depend on an increase in the erythrocyte
cytoplasmic calcium, since most of the calcium was shown to be located in the parasite.

Plasmodium falciparum - malaria - erythrocyte - transglutaminase - calcium

© Copyright 1999 Fundacao Oswaldo Cruz - Fiocruz
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