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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 94, No. 2, 1999, pp. 245-248
Bioline Code: oc99048
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 2, 1999, pp. 245-248

 en Review on the Molecular Tools for the Understanding of the Epidemiology of Animal Trypanosomosis in West Africa
G Duvallet; S de La Rocque; JM Reifenberg; P Solano; T Lefrançois; JF Michel; Z Bengaly; I Sidibe; D Cuisance & G Cuny

Abstract

The epidemiology of animal trypanosomosis around Bobo-Dioulasso (Burkina Faso, West Africa) benefited a lot in the last years from the progress of molecular tools. The two most used molecular techniques were the polymerase chain reaction for the diagnosis of the disease in cattle and the characterization of the
trypanosomes in the host and the vector on one hand, and the microsatellite DNA polymorphism in tsetse flies to study the intraspecific genetic variability of the vector on the other hand. The results obtained in the Sideradougou area during a recent two year survey with these techniques, associated with many other
georeferenced informations concerning vector and cattle distribution, natural environment, landuse, ground occupation, livestock management, were combined in a Geographical Information System. This new approach of a complex pathogenic system led to a better evaluation of the risk of trypanosome transmission.

Keywords
African animal trypanosomosis - epidemiology - polymerase chain reaction- microsatellite DNA - Glossina - Trypanosoma sp.

 
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