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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 94, No. 3, 1999, pp. 323-328
Bioline Code: oc99061
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 3, 1999, pp. 323-328

 en Restriction Fragment Length Polymorphism of 195 bp Repeated Satellite DNA of Trypanosoma cruzi Supports the Existence of Two Phylogenetic Groups
Brigitte Bastrenta; Marie France Bosseno; Christian Barnabé; Michel Tibayrenc & Simone Frédérique Brenière

Abstract

The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf I) and high molecular weight DNA (Hae III), while group 2 presents a ladder profile for each enzyme, which is a characteristic of tandemly repeated DNA. The two groups, respectively, clustered stocks pertaining to the two principal lineages evidenced by isoenzyme and RAPD markers. The congruence among these three independent genomic markers corroborates the existence of two real phylogenetic lineages in T. cruzi. The specific monomorphic profiles for each major phylogenetic lineage suggest the existence of ancient sexuality and cryptic biological speciation.

Keywords
Trypanosoma cruzi - satellite DNA - 195 bp repeat unit

 
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