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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 94, No. 3, 1999, pp. 367-370
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Bioline Code: oc99068
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 3, 1999, pp. 367-370
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T Cell Response of Asymptomatic Leishmania chagasi Infected Subjects to Recombinant Leishmania Antigens
Sérgio Ricardo Costa; Argemiro D'Oliveira Júnior; Olívia Bacellar & Edgar M Carvalho
Abstract
In areas of Leishmania chagasi transmission the ability to control leishmania infection is associated with IFN-γ production. In visceral leishmaniasis down-regulation of T cell
responses is mediated by interleukin-10 (IL-10). In this study we evaluated the lymphoproliferative response, IFN-γ and IL-10 production on lymphocyte cultures stimulated with recombinant leishmania antigens in subjects with asymptomatic L. chagasi infection. There was a statistically significant
difference in the lymphoproliferative response of the subjects with asymptomatic infection as compared to patients with visceral leishmaniasis and healthy subjects with respect to crude antigens (p<0.01), gp-63 (p<0.05) and hsp-70 (p<0.01), as well as between asymptomatic L. chagasi infected subjects and patients with visceral leishmaniasis with respect to the response to all antigens tested. The IFN-γ production observed in the group with asymptomatic infection with all the three recombinant antigens tested was higher (p<0.01) than that observed in patients with visceral leishmaniasis and in
healthy subjects. Furthermore, in individuals with asymptomatic infection, IL-10 levels in cultures stimulated with recombinant antigens were very low. This study shows that lymphocytes from individuals with asymptomatic L. chagasi infection are able to recognize recombinant leishmania antigens with production of a cytokine that is associated with leishmania killing.
Keywords
asymptomatic Leishmania chagasi infection - recombinant leishmania antigens - T cell responses
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