Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
Vol. 94, No. 4, 1999, pp. 491-496
Bioline Code: oc99090
Full paper language: English
Document type: Research Article
Document available free of charge
Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 4, 1999, pp. 491-496
© Copyright 1999 Fundacao Oswaldo Cruz - Fiocruz
A dhfr-ts-Leishmania major Knockout Mutant Cross-protects against Leishmania amazonensis|
PST Veras; CI Brodskyn; FMP Balestieri; LAR de Freitas; APS Ramos; ARP Queiroz; A Barral; SM Beverley & M Barral-Netto
E10-5A3 is a dhfr-ts- Leishmania major double knockout auxotrophic shown previously to induce substantial protection against virulent L. major infection in both genetically susceptible and resistant mice. We investigated the capacity of dhfr-ts- to protect against heterologous infection by L. amazonensis. The degree of protection was evaluated by immunization of BALB/c or C57BL/6 mice with E10-5A3, followed by L. amazonensis challenge. Whether immunized by subcutaneous (SC) or intravenous (IV) inoculation, susceptible and resistant mice displayed a partial degree of protection against challenge with virulent L. amazonensis. SC-immunized BALB/c mice developed lesions 40 to 65% smaller than non immunized mice, while IV immunization led to protection ranging from 40 to 75% in four out of six experiments compared to non immunized animals. The resistant C57BL/6 mice displayed comparable degrees of protection, 57% by SC and 49% by IV immunization.
Results are encouraging as it has been previously difficult to obtain protection by SC vaccination against Leishmania, the preferred route for human immunization.
auxotrophic Leishmania major - Leishmania amazonensis - immunization - cross-protection
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