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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 94, No. 6, 1999, pp. 763-770
Bioline Code: oc99226
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 6, 1999, pp. 763-770

 en Extracellular Serine-proteinases Isolated from Streptomyces alboniger: Partial Characterization and Effect of Aprotinin on Cellular Structure
Lopes, Andréa; Coelho, Rosalie R. R.; Meirelles, Maria Nazareth L; Branquinha, Marta Helena & Vermelho, Alane Beatriz

Abstract

Streptomyces alboniger ATCC 12461 grown in brain heart infusion (BHI) medium produced two extracellular serine-proteinases, denoted SP I and SP II, which were purified by ammonium sulfate precipitation and aprotinin-agarose affinity chromatography. SP I was purified 88,9-fold and SP II 66,7- fold, with 33.4% and 10.4% yield, respectively. The optimum pH for the proteinases activity, using a-N-p-tosyl-L-arginine-methyl ester (TAME) as substrate, was 9-10 and the optimum temperature was 37C. The proteolytic activity of SP I and SP II was inhibited by aprotinin and SP I was partially inhibited by leupeptin, both serine-proteinase inhibitors. S. alboniger growth in BHI-liquid medium decreased when 5 microgram/ml, 10 microgram/ml of aprotinin was used, being completely inhibited with 20 microgram/ml and 40 microgram/ml. At the ultrastructural level, aprotinin-treated S. alboniger cells showed swelling of the bacterial body and condensation of the genetic material, probably related to the inhibition of its growth.

Keywords
Streptomyces alboniger - serine-proteinases - growth - aprotinin

 
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