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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN: 1677-3225
Vol. 5, No. 17, 2006, pp. 1034-1040
Bioline Code: os06020
Full paper language: English
Document type: Research Article
Document available free of charge

Brazilian Journal of Oral Sciences, Vol. 5, No. 17, 2006, pp. 1034-1040

 en P53 gene mutation and protein expression in ameloblastomas
K. A. Al-Salihi; Ling Yoke Li & Azlina A.

Abstract

P53 gene is commonly found to be mutated in human cancer. Currently, limited data exist on the occurrence of p53 gene mutation in ameloblastomas. This study designed to evaluate the expression of p53 proteins and their gene mutations in human ameloblastomas. Four cases of epithelial odontogenic tumors were used. Normal cells of a 7 months aborted fetus's mandible were used as negative control. P53 Protein expression was detected immunohistochemically. DNA was extracted and amplified using PCR. Gene sequences analyzed to determine p53 mutation. p53 was overexpressed in one case of unicystic ameloblastoma and 2 cases of variant types of ameloblastoma. The Coding sequencing analyses demonstrated p53 gene alteration in cases of ameloblastoms in Exon 7 while, fetus epithelial mucosal cells showed the wild type DNA sequence in Exon 4. In conclusion p53 protein overexpression was detected by immunohistochemical staining as well as by DNA sequence analysis in cases of ameloblastomas.

Keywords
ameloblastoma, p53, immunohistochemistry, mandible

 
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