An in vitro microbial model for producing caries-like lesions on enamel|
Steiner-Oliveira, Carolina; Maciel, Fernando A.; Rodrigues, Lidiany K.A.; Napimoga, Marcelo H.; Pimenta, Luiz A.F.; Höfling, José F. & Gonçalves, Reginaldo B.
This study aimed to develop a low cost in vitro viable microbiological
model to produce biofilms to be used in dental researches. Single and
multi-species biofilms of S. mutans, S. sobrinus, S. mitis, S. salivarius,
S. cricetus and S. sanguinis were grown on bovine enamel slabs during
10 days, in a sterile brain-heart infusion broth, containing 5% sucrose
and incubated at 37°C in an atmosphere of 10% CO2. The slabs were
transferred to a fresh medium at every 6, 12 or 24 hours. After the
experimental period, enamel volume percent mineral was determined
by cross-sectional microhardness. Caries-like lesions were found in
all bacterial groups when compared with the control group. No
statistical significant differences were found between S. mutans and S.
sobrinus with respect of their cariogenicity or among the periods of
medium change. However, it was found a statistical significant
difference among the cariogenicity of S. salivarius and S. sanguinis
(ANOVA followed by Tukey test). This model has successfully
developed caries-like lesion on enamel and the medium can be changed
at every 24 hours utilizing either S. mutans or S. sobrinus.
biofilms, microbiological, enamel, S. mutans, S. sobrinus