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Effect of storage and disinfection methods of extracted bovine teeth on bond strength to dentin
Humel, Maria Malerba Colombi; Oliveira, Marcelo Tavares; Cavalli, Vanessa & Giannini, Marcelo
Abstract
The aim of this study was to evaluate the effect of storage and
disinfection methods (SDM) on bond strength (BS) to bovine dentin,
using two adhesive systems: Adper Single Bond and Clearfil Protect
Bond. Method: Extracted bovine teeth were assigned to the following
SDM: 100% Humidity (HU); Gamma Radiation (GR); Autoclave
(AU); 0.10g/mL Thymol (TH); 10% Formalin (FO); Frozen (FR);
0.2% Sodium Azide (SA) and 0.5% Chloramine T (CT) (n=10). The
GR and AU groups were submitted to sterilization methods and
stored at 100% humidity for 24 hours at 37C°, before testing. TH,
FO, FR, SA and CT groups were stored for three months at 5C°,
except for FR (-4C°). The adhesive systems were applied according
to manufacturers instructions. For BS testing, the micro-shear test
was performed, using Tygon mold (0.75mm diameter and 1mm high),
which was filled with Clearfil AP-X (Kuraray) composite resin.
Data were analyzed by two-way ANOVA and Tukey Test (p<0.05).
BS of adhesive systems was similar for all SDM, except for 10%
formalin. HU, GR, AU, TH and SA did not change the BS for the
adhesives tested. For most of SDM, BS of adhesive systems was
similar. Some 3 month-SDM groups (FR, FO and CT) reduced the
BS.
Keywords
dentin, disinfection, sterilization, dentin-bonding agents
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