Cytotoxicity of intraoral orthodontic elastics|
Santos, Rogério Lacerda Dos; Pithon, Matheus Melo; de Oliveira, Márlio Vinícius de; Mendes, Gabriella da Silva; Romanos, Maria Teresa Villela & Ruellas, Antônio Carlos de Oliveira
The present study compared in vitro four types of latex intraoral
elastics (5/16 = 7.9 mm, mean load) regarding their possible cytotoxic
Material and Methods:
The sample was allocated to four
groups of 24 elastics: Group A (American Orthodontics), Group M
(Morelli), Group T (TP Orthodontics), and Group U (Uniden).
Cytotoxicity assays were performed using cell culture medium
containing epithelioid-type cells (Hep-2 line) derived from human
laryngeal carcinoma. Two methods for evaluating the cytotoxicity,
the agar overlay test and "dye-uptake" test, were employed at two
different moments (0 and 24 h). Data were compared by analysis of
variance (ANOVA) and Tukey's test (p<0.05).
The results showed statistically significant difference (p<0.05) between Groups
T and M, T and U, A and M, and A and U, for dye-uptake test at 0
and 24 h. No cell lysis was found in Groups T and A for the agar
overlay test, whereas 80% cell lysis was found in Groups M and U
after 24 h according to the Stanford's response index (RI).
Both TP Orthodontics and American Orthodontics
elastics evoked less cell lysis than Morelli and Uniden elastics.
cytotoxicity; elastics; biocompatibility; orthodontics