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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN: 1677-3225
Vol. 7, No. 25, 2008, pp. 1531-1534
Bioline Code: os08012
Full paper language: English
Document type: Research Article
Document available free of charge

Brazilian Journal of Oral Sciences, Vol. 7, No. 25, 2008, pp. 1531-1534

 en Phenotypic characterization of Candida check for this species in other resources spp. isolates from chronic periodontitis patients
Koga-Ito, Cristiane Yumi; Komiyama, Edson Yukio; de Paiva Martins, Clélia Aparecida; dos Santos, Silvana Soléo Ferreira; Balducci, Ivan & Jorge, Antonio Olavo Cardoso

Abstract

Aim: Several typing methods for Candida check for this species in other resources spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals.
Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans , the most frequently observed being 0000 and 0001.
Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups.
Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients.

Keywords
periodontitis, Candida albicans, biotyping, morphotyping

 
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