Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
Vol. 11, No. 3, 2012, pp. 422-427
Bioline Code: os12045
Full paper language: English
Document type: Research Article
Document available free of charge
Brazilian Journal of Oral Sciences, Vol. 11, No. 3, 2012, pp. 422-427
© Copyright 2012 - Braz J Oral Sci.
Functional activity of neutrophils and systemic inflammatory response of Down’s syndrome patients with periodontal disease|
Freire, Isabelle Rodrigues; Aguiar, Sandra Maria Herondina Coelho Ávila & de Oliveira, Sandra Helena Penha
Periodontal disease (PD) is characterized as an inflammatory process that compromises the
support and protection of the periodontium. Patients with Down’s syndrome (DS) are prone to
develop PD. Neutrophils (NE) are the first line of defense against infection and their absence sets
the stage for disease. Aim: To compare the activity and function of NE in the peripheral blood from
DS patients with and without PD, assisted at the Center for Dental Assistance to Patients with
Special Needs affiliated with the School of Dentistry of Araçatuba, Brazil. Methods: Purified NE
were collected from peripheral blood of 22 DS patients. NE were used to detect the 5-lypoxigenase
(5-LO) expression by RT-PCR. Plasma from peripheral blood was collected to measure tumor
necrosis factor-a (TNF-α) and interleukin-8 (IL-8) by ELISA and nitrite (NO3) using a Griess
assay. Results: Data analysis demonstrated that DS patients with PD present high levels of
TNF-a and IL-8 when compared with DS patients without PD. However, there was no statistically
significant difference in the levels of NO3 production between the groups. The levels of the
inflammatory mediator 5-LO expression increased in DS patients with PD. Conclusions: According
with these results, it was concluded that TNF-α and IL-8 are produced by DS patients with PD.
Furthermore, DS patients with PD presented high levels of 5-LO expression, suggesting the
presence of leukotriene B4 (LTB4) in PD, thus demonstrating that the changes in NE function due
to the elevation of inflammatory mediators contribute to PD.
Down syndrome, periodontal disease, 5-lypoxigenase, tnf-a.
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