A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth|
Leal Silva, Emmanuel João Nogueira; Bluzarca Rollemberg, Carolina; de Souza Coutinho-Filho, Tauby; Liess Krebs, Renato & Augusto Zaia, Alexandre
Aim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic
solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis
employing 3T3 cells.
Methods: Plates containing confluent 3T3 fibroblasts were exposed to the
various media for 24h, at 37°C with 5% CO2, and cell viability was evaluated by a multiparametric
assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity
(neutral red test), and total cell density (crystal violet dye exclusion test). Results from each test
were compared by one-way analysis of variance (ANOVA).
Results: Statistical analysis showed
that whole milk and HBSS were the most effective media in maintaining cell viability at all tested
times (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects.
study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3
fibroblasts as demonstrated by three different cell viability tests.
tooth avulsion, fibroblasts, cell viability