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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN:
Vol. 12, No. 2, 2013, pp. 143-147
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Bioline Code: os13029
Full paper language: English
Document type: Research Article
Document available free of charge
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Brazilian Journal of Oral Sciences, Vol. 12, No. 2, 2013, pp. 143-147
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Proliferation of human periodontal ligament mesenchymal cells on polished and plasma nitriding titanium surfaces
Alves Ribeiro, Rodrigo; Gadelha Vasconcelos, Rodrigo; Ginani, Fernanda; da Silva, José Sandro Pereira; Alves-Júnior, Clodomiro & Barboza, Carlos Augusto Galvão
Abstract
Aim: To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal
ligament on polished and plasma-treated titanium surfaces. Methods: Eighteen titanium disks were
polished and half of them (n=9) were submitted to plasma nitriding using the cathodic cage technique.
Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and
cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell
proliferation. Cell proliferation was analyzed and growth curves were constructed for the different
groups by determining the number of cells adhered to the different surfaces at 24, 48 and 72 h after
plating. Results: Higher cell number was observed for the nitrided surface at 24 and 48 h.
However, no statistically significant difference in cell proliferation was observed between the two
different surface treatments (p>0.05). Conclusions: We concluded that plasma nitriding produced
surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. Associated
to other physical and chemical properties, it is possible to assume the feasibility of plasma nitriding
method and its positive effect on the early cellular events of osseointegration.
Keywords
biocompatible materials, titanium, cell proliferation, periodontal ligament
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