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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN: 1677-3225
Vol. 12, No. 3, 2013, pp. 199-204
Bioline Code: os13039
Full paper language: English
Document type: Research Article
Document available free of charge

Brazilian Journal of Oral Sciences, Vol. 12, No. 3, 2013, pp. 199-204

 en Correlation of phospholipase and proteinase production of Candida check for this species in other resources with in vivo pathogenicity in Galleria mellonella check for this species in other resources
Rossoni, Rodnei Dennis; Barbosa, Júnia Oliveira; Vilela, Simone Furgeri Godinho; dos Santos, Jéssica Diane; Jorge, Antonio Olavo Cardoso & Junqueira, Juliana Campos


An essential factor to the virulence of the genus Candida check for this species in other resources is the ability to produce enzymes and this may be crucial in the establishment of fungal infections.
Aim: This study investigated in vitro enzymatic activities of Candida species and their virulence in an in vivo Galleria mellonella check for this species in other resources experimental model.
Methods: Twenty-four clinical strains of Candida spp. isolated from the human oral cavity were evaluated, including the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. norvegensis, C. lusitaniae and C. guilliermondii. All Candida strains were tested in vitro for production of proteinase and phospholipase. The Candida strains were also injected into Galleria mellonella larvae to induce experimental candidiasis, and after 24 hours, the survival rate was assessed.
Results: Phospholipase and proteinase activity were observed in 100% of the C. albicans strains. In the non-albicans species, proteinase and phospholipase activity were observed in 25 and 43% of the studied strains, respectively. The most pathogenic Candida species in G. mellonella were C. albicans, C. dubliniensis and C. lusitaniae, whereas C. glabrata was the least virulent species. Furthermore, a positive significant correlation was found between both enzymatic activities with virulence in G. mellonella.
Conclusions: The virulence of Candida strains in G. mellonella is related to the quantity of proteinases and phospholipases production of each strain.

Candida; virulence factors; invertebrates

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