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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN:
Vol. 13, No. 1, 2014, pp. 22-27
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Bioline Code: os14005
Full paper language: English
Document type: Research Article
Document available free of charge
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Brazilian Journal of Oral Sciences, Vol. 13, No. 1, 2014, pp. 22-27
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Effects of various hydrogen peroxide bleaching concentrations and number of applications on enamel
Bittencourt Berger, Sandrine; Eduardo Silva Soares, Luis; Abrahão Martin, Airton; Ambrosano, Gláucia Maria Bovi; Tabchoury, Cínthia Pereira Machado & Giannini, Marcelo
Abstract
Aim: To evaluate the effects of three hydrogen peroxide (HP) concentrations on enamel mineral
content (MC), following three peroxide applications and using three methods to measure the MC.
Methods: Forty samples were obtained from bovine incisors and randomly divided into 4 groups
(n=10). The control group remained untreated, while the experimental groups were tested for three HP
concentrations (10%, 35% and 50%). The HP gel was applied 3 times on the enamel surface for 30
min per application and the samples were analyzed after each application. The MC of the enamel was
determined before and after bleaching using Fourier transform (FT-Raman) spectroscopy and micro
energy-dispersive x-ray fluorescence spectrometry (μEDXRF). The calcium (Ca) lost from the bleached
enamel was quantified with an atomic absorption spectrometer (AAS). The data were analyzed statistically
by ANOVA, Tukey and Dunnett´s tests (p≤0.05). Results: The FT-Raman showed a decrease in MC
for all bleaching treatments, without influence of the different HP concentrations or the number of
applications. μsEDXRF did not detect any changes in MC. Conclusions: Ca loss was observed by the
AAS, with no difference among the three HP concentrations. The FT-Raman and AAS analyses
detected MC reduction and Ca loss after HP bleaching.
Keywords
dental enamel; bleaching agents; hydrogen peroxide
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© Brazilian Journal of Oral Sciences Alternative site location: http://www.fop.unicamp.br/brjorals
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