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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN: 1677-3225
Vol. 13, No. 1, 2014, pp. 53-57
Bioline Code: os14011
Full paper language: English
Document type: Research Article
Document available free of charge

Brazilian Journal of Oral Sciences, Vol. 13, No. 1, 2014, pp. 53-57

 en In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms
de Oliveira, Bruna Paloma; dos Santos Accioly Lins, Carla Cabral; Diniz, Fátima Alves; Melo, Liliane Lima & de Castro, Célia Maria Machado Barbosa

Abstract

Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans check for this species in other resources , Pseudomonas aeruginosa check for this species in other resources , Enterococcus faecalis check for this species in other resources and Staphylococcus aureus check for this species in other resources . A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 μM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 μL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane’s comparisons. Results: The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.

Keywords
endodontics; Enterococcus faecalis; methylene blue; microbiology; photodynamic therapy

 
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