Resin-based sealer penetration into dentinal tubules after the use of 2% chlorhexidine gel and 17% EDTA: in vitro study|
Sabadin, Natalia; Böttcher, Daiana Elisabeth; Hoppe, Carolina Bender; dos Santos, Régis Burmeister & Grecca, Fabiana Soares
Aim: To evaluate, by confocal laser scanning microscopy (CLSM), the influence of 17% EDTA
final irrigation on the penetration of an endodontic resin-based sealer into dentinal tubules after
use of 2% chlorhexidine gel. Methods: Forty extracted bovine incisors were instrumented
according to the groups: G1 – root canal preparation with 2% chlorhexidine gel (n=10); G2 –
root canal preparation with 2% chlorhexidine gel and final irrigation with 17% EDTA (n=10); G3
– root canal preparation with saline and final irrigation with 17% EDTA (n=10); G4 – root canal
preparation with saline (n=10). The samples were filled with gutta-percha using AH Plus sealer
with rhodamine B fluorescent dye. After seven days, the teeth were sectioned at the coronal,
middle, and apical thirds and viewed under confocal microscope. The most representative area
of penetration depth was measured in each group. Statistical significance for the sealer penetration
area was determined among groups using one-way ANOVA followed by Tuckey test. For thirds
comparison, in each group, data were statistically analyzed using Friedman test (p<0.05).
Results: The maximum penetration was provided by G2 - 2% chlorhexidine + EDTA (p=0.000).
According to this criterion, no differences were found among the other groups and among thirds
within the same group. Conclusions: Based on these results, the use of 17% EDTA should be
indicated after root canal preparation with 2% chlorhexidine gel for smear layer removal,
enhancing the AH Plus sealer penetration.
endodontics; root canal filling; smear layer; in vitro