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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN: 1677-3225
Vol. 14, No. 2, 2015, pp. 135-140
Bioline Code: os15023
Full paper language: English
Document type: Research Article
Document available free of charge

Brazilian Journal of Oral Sciences, Vol. 14, No. 2, 2015, pp. 135-140

 en Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
Taha, Siti Aisyah Mohd; Ling, Joanne Koh Su; Binti Azmi, Nur Izyan; Kannan, Thirumulu Ponnuraj; Azlina, Ahmad & Mokhtar, Khairani Idah

Abstract

Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7. Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.

Keywords
receptors; notch; gene expression; stem cells; tooth; deciduous; culture media

 
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