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Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN: 1677-3225
Vol. 14, No. 2, 2015, pp. 149-153
Bioline Code: os15025
Full paper language: English
Document type: Research Article
Document available free of charge

Brazilian Journal of Oral Sciences, Vol. 14, No. 2, 2015, pp. 149-153

 en Agreement between RAPD, API20C AUX, CHROMagar Candida and microculture on oral Candida identification
Pires, Emanuene Galdino; de Freitas, Edimilson Martins; Bonan, Paulo Rogério Ferreti & Nobre, Sérgio Avelino Mota

Abstract

Aim: To measure the agreement of methods for identification of Candida check for this species in other resources species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. After this, agreement among methods using Kappa test was performed. Results: Agreement rates between RAPD and CHROMagar Candida, showed significant accuracy for C. albicans check for this species in other resources , C. tropicalis check for this species in other resources , C. dubliniensis check for this species in other resources and C. krusei check for this species in other resources (Kappa: 0.760, 0.640, 0.416 and 0.360, respectively, p<0.05). Comparing RAPD results with microculture, the highest agreement was for C. albicans (Kappa: 0.851 - p<0.05) but no significant agreement for C. lusitaniae check for this species in other resources , C. krusei and C. guilliermondii check for this species in other resources was obtained (p>0.05). The agreement was significant for all identified species when RAPD (OPE-18) and API 20C AUX (p<0.05) were used. Critical levels of agreement between RAPD and microculture were observed when C. lusitaniae, C. krusei and C. guilliermondii were identified. Conclusions: API 20C AUX presented the best agreement with molecular random identification and CHROMagar showed good agreement for C. albicans, C. tropicalis, C. dubliniensis and C. krusei identification.

Keywords
candida; mouth; methods

 
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