search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Brazilian Journal of Oral Sciences
Piracicaba Dental School - UNICAMP
EISSN: 1677-3225
Vol. 14, No. 3, 2015, pp. 240-245
Bioline Code: os15043
Full paper language: English
Document type: Research Article
Document available free of charge

Brazilian Journal of Oral Sciences, Vol. 14, No. 3, 2015, pp. 240-245

 en Enterococcus spp. check for this species in other resources isolated from root canals with persistent chronic apical periodontitis in a Chilean population
Sánchez-Sanhueza, Gabriela; González-Rocha, Gerardo; Dominguez, Mariana & Bello-Toledo, Helia

Abstract

Aim: To isolate and identify in a Chilean population, Enterococcus spp. check for this species in other resources from root canals with persistent chronic apical periodontitis (CAP) and to investigate the potential correlation between the bacteria and the observed clinical features. Methods: Twenty patients with indication for endodontic retreatment due to persistent CAP were selected. Data from patient general health and dental clinical history were recorded. During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. The relationship between the number of colony-forming units of Enterococcus spp. and patient clinical data was assessed statistically by the Pearson Chi square and Fisher exact tests. Finally, a Polymerase Chain Reaction (PCR) assay to determine the most prevalent species of Enterococcus spp. was conducted in the clinical samples, and the results were analyzed by a proportion comparison test. Results: Enterococcus spp. strains were isolated in 70% of the patients. Most of them (98.8%) accounted for Enterococcus faecalis check for this species in other resources and only 1.2% for Enterococcus faecium check for this species in other resources . A high frequency of E. faecalis was found in teeth with inadequate endodontic treatment or dental crown restorations. Conclusions: This study concluded that E. faecalis is prevalent in root canals with persistent CAP in a Chilean population. E. faecium as found in a single case with the poorest root canal filling. Further studies are still required to investigate the presence of other species, which may be linked to persistent chronic apical periodontitis.

Keywords
periapical granuloma; Enterococcus faecalis; Enterococcus

 
© Copyright 2015 - Brazilian Journal of Oral Sciences
Alternative site location: http://www.fop.unicamp.br/brjorals

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil