Iranian Journal of Pediatrics
Tehran University of Medical Sciences Press
Vol. 21, No. 3, 2011, pp. 278-286
Bioline Code: pe11048
Full paper language: English
Document type: Research Article
Document available free of charge
Iranian Journal of Pediatrics, Vol. 21, No. 3, 2011, pp. 278-286
© Copyright 2011 Iran Journal of Pediatrics.
Evaluation of Serum and Pleural Levels of Angiopoietin-1 and Angiopoietin- 2 in Children with Transudative and Exudative Pleural Effusions|
Sanad, Mohammed; Shouman, Waheed & Gharib, Amal F.
Objective: Angiopoietins are involved in the pathogenesis of a variety of human diseases. We tried
to evaluate the application of pleural and serum Angiopoietin-1 and 2 in categorizing pleural
effusions (PEs) into exudates and transudates in children.
Methods: Pleural fluid (PF) and serum Angiopoietin (Ang)-1 and Ang-2 were measured in 80
children with PEs (40 transudative and 40 exudative) by using enzyme-linked immunosorbent
Findings: PF Ang-2 levels were significantly higher in pleural exudates than in transudates (P
0.012). PF Ang-2 levels were significantly higher than serum Ang-2 levels in patients with pleural
exudates and transudates (P<0.001). PF Ang-2 levels were higher in tuberculous than in nontuberculous
pneumonic PEs and empyema (P=0.01). PF Ang-2 levels correlate with serum Ang-2
levels (P<0.003). PF Ang-1 levels were significantly lower than serum Ang-1 levels both in patients
with exudates and those with transudates (P<0.001). Cutoff points of serum and PF Ang-2,
differentiating between transudative and exudative effusions were 3ng/ml and 8ng/ml
respectively. Predictive potentials of serum and PF Ang-2 cutoff points were: Sensitivity 90% and
95% respectively, specificity 92.50% and 97.50% respectively, positive predictive value 92.30%
and 97.40% respectively and negative predictive value 90.20% and 95.10% respectively.
Conclusion: Ang-2 levels were elevated in exudative PEs and correlated with levels of markers of
pleural inflammation and pleural vascular hyperpermeability. It could categorize PE to exudates
and transudates with valuable discriminative properties. That was detected more obviously in
pleural fluids than in serum.
Pleural Effusions; Transudates; Exudates; Angiopoietin-1; Angiopoietin-2
Alternative site location: http://diglib.tums.ac.ir/pub/