Iranian Journal of Pediatrics
Tehran University of Medical Sciences Press
Vol. 23, No. 4, 2013, pp. 451-457
Bioline Code: pe13085
Full paper language: English
Document type: Research Article
Document available free of charge
Iranian Journal of Pediatrics, Vol. 23, No. 4, 2013, pp. 451-457
© Iranian Journal of Pediatrics
Expression of Activation-Induced Cytidine Deaminase Gene in B Lymphocytes of Patients with Common Variable Immunodeficiency|
Abolhassani, Hassan; Farrokhi, Amir Salek; Pourhamdi, Shabnam; Mohammadinejad, Payam; Sadeghi, Bamdad; Moazzeni, Seyed-Mohammad & Aghamohammadi, Asghar
Objective: Common variable immunodeficiency (CVID) is a heterogeneous disorder characterized by reduced
serum level of IgG, IgA or IgM and recurrent bacterial infections. Class switch recombination (CSR) as a critical
process in immunoglobulin production is defective in a group of CVID patients. Activation-induced cytidine
deaminase (AID) protein is an important molecule involving CSR process. The aim of this study was to
investigate the AID gene mRNA production in a group of CVID patients indicating possible role of this
molecule in this disorder.
Methods: Peripheral blood mononuclear cells (PBMC) of 29 CVID patients and 21 healthy controls were
isolated and stimulated by CD40L and IL-4 to induce AID gene expression. After 5 days AID gene mRNA
production was investigated by real time polymerase chain reaction.
Findings: AID gene was expressed in all of the studied patients. However the mean density of extracted AID
mRNA showed higher level in CVID patients (230.95±103.04 ng/ml) rather than controls (210.00±44.72
ng/ml; P=0.5). CVID cases with lower level of AID had decreased total level of IgE (P=0.04) and stimulated IgE
production (P=0.02); while cases with increased level of AID presented higher level of IgA (P=0.04) and
numbers of B cells (P=0.02) and autoimmune disease (P=0.02).
Conclusion: Different levels of AID gene expression may have important roles in dysregulation of immune
system and final clinical presentation in CVID patients. Therefore investigating the expression of AID gene can
help in classifying CVID patients.
Activation-Induced Cytidine Deaminase; AICDA Protein; Common Variable Immunodeficiency; Class Switching
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