Objective: To evaluate the
in vivo and
in vitro activity of Canova in experimental infection with
Paracoccidioides brasiliensis
.
Materials and Methods: Mice infected with
P. brasiliensis were treated with Canova for 17 weeks. Follow-up measures included the determination of total antibodies, global and differential leukocyte counts. Further, nitric oxide production was determined by adding macrophage cultures to different concentrations of Canova in the presence or absence of
P. brasiliensis.
Results: The data revealed the protective effect of Canova in
P. brasiliensis- infected animals. A higher nitric oxide production was found in the Canova- treated cultures.
Conclusion: These data suggest that Canova activates the macrophages by a way that depends, at least in part, on nitric oxide.
