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Indian Journal of Pharmacology
Medknow Publications on behalf of Indian Pharmacological Society
ISSN: 0253-7613
EISSN: 1998-3751
Vol. 40, No. 5, 2008, pp. 227-229
Bioline Code: ph08064
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Pharmacology, Vol. 40, No. 5, 2008, pp. 227-229

 en Preliminary Isolation and in vitro Antiyeast Activity of Active Fraction from Crude Extract of Gracilaria changii check for this species in other resources
Sasidharan, Sreenivasan; Darah, Ibrahim & Noordin Mohd Kassim, Mohd Jain

Abstract

Objective: To isolate the active fraction from crude extract of Gracilaria changii and to determine its in vitro antifungal activity.
Materials and Methods: The active fraction was isolated from the crude extract of G. changii by various purification procedures such as column chromatography, thin layer chromatography, bioauthograph etc. The in vitro antifungal activity ( Candida albicans check for this species in other resources ) of the active fraction (1.00, 0.50, and 0.25 mg/ml) was studied by disc diffusion method and the effect of the active fraction on the morphology of yeast was done by scanning electron microscope (SEM) studies.
Results: An active fraction with remarkable antifungal activity was separated from the crude extract. The active fraction was effective as a fungicide against C. albicans and showed a dose-dependent antifungal activity. A Scanning Electron Microscope (SEM) study confirmed the fungicidal effect of G. changii active fraction on C. albicans, by changing the normal morphology of C. albicans.
Conclusion: From G. changii crude extract, an active fraction with remarkable in vitro antifungal activity has been isolated.

Keywords
Antiyeast activity, candida albicans, Gracilaria changii, marine algae

 
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