search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Indian Journal of Pharmacology
Medknow Publications on behalf of Indian Pharmacological Society
ISSN: 0253-7613
EISSN: 1998-3751
Vol. 42, No. 2, 2010, pp. 65-69
Bioline Code: ph10022
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Pharmacology, Vol. 42, No. 2, 2010, pp. 65-69

 en Immunomodulatory activity of a Chinese herbal drug Yi Shen Juan Bi in adjuvant arthritis
Perera, Pathirage Kamal; Li, Yunman; Peng, Cheng; Fang, Weirong & Han, Caifeng

Abstract

Objective: To investigate the immunomodulating mechanisms of a Chinese herbal medicine Yi Shen Juan Bi (YJB) in treatment of adjuvant arthritis (AA) in rats.

Materials and Methods: Levels of serum tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β) were measured by the Enzyme-Linked Immunosorbent Assay (ELISA). Expression of TNF-α mRNA and IL-1β mRNA in synovial cells was measured with the semi-quantitative technique of reverse transcription-polymerase chain reaction (RT-PCR), while caspase-3 was examined by western blot analysis.

Results: The administration of YJB significantly decreased the production of serum TNF-α and IL-1β. It also decreased significantly the TNF-α mRNA, IL-1β mRNA, and caspase-3 expression in synoviocytes.

Conclusions: YJB produces the immunomodulatory effects by downregulating the over-activated cytokines, while it activates caspase-3, which is the key executioner of apoptosis in the immune system. This may be the one of the underlying mechanisms that explains how YJB treats the rheumatoid arthritis.

Keywords
Synoviocytes, TNF-α, IL-1β, caspase-3

 
© Copyright 2010 Indian Journal of Pharmacology.
Alternative site location: http://www.ijp-online.com

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2020, Site last up-dated on 07-Jul-2020.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil