search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Indian Journal of Pharmacology
Medknow Publications on behalf of Indian Pharmacological Society
ISSN: 0253-7613
EISSN: 1998-3751
Vol. 42, No. 2, 2010, pp. 99-104
Bioline Code: ph10030
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Pharmacology, Vol. 42, No. 2, 2010, pp. 99-104

 en In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata check for this species in other resources leaves
Raj, Vasanth P.; Chandrasekhar, Raghu H.; Vijayan, P.; Dhanaraj, S. A.; Rao, Mallikarjuna C.; Rao, Venkata J. & Nitesh, K.

Abstract

Objective: To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata check for this species in other resources for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models.

Materials and Methods: Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of the total alkaloid fraction (80-40 μg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150-200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30% CCl4 suspended in olive oil (1 ml/kg body weight, i.p).

Results: The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80-40 μg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight.

Conclusion: The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.

Keywords
HepG2, hepatocytes, hepatoprotective, Hygrophila auriculata, total alkaloidsIntroduction

 
© Copyright 2010 Indian Journal of Pharmacology.
Alternative site location: http://www.ijp-online.com

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2020, Site last up-dated on 07-Jul-2020.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil