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Indian Journal of Pharmacology
Medknow Publications on behalf of Indian Pharmacological Society
ISSN: 0253-7613
EISSN: 1998-3751
Vol. 42, No. 2, 2010, pp. 105-107
Bioline Code: ph10031
Full paper language: English
Document type: Short Communication
Document available free of charge

Indian Journal of Pharmacology, Vol. 42, No. 2, 2010, pp. 105-107

 en In vitro antibacterial evaluation of Anabaena check for this species in other resources sp. against several clinically significant microflora and HPTLC analysis of its active crude extracts
Chauhan, Abhishek; Chauhan, Garima; Gupta, Prakash C.; Goyal, Pankaj & Kaushik, P.

Abstract

The present study was conducted to evaluate the possible antibacterial activity of Anabaena check for this species in other resources extracts. Anabaena was isolated from a natural source and cultured in vitro. After suitable growth, cyanobacterial culture was harvested using different solvents. Extracts, thus prepared, were evaluated for their antibacterial potential by agar-well diffusion assay against bacterial species of clinical significance. MIC values were determined further to check the concentration ranges for significant inhibition. HPTLC analysis was done to separate the components of active crude extract in an attempt to identify the bio-active chemical entity. Methanol extract exhibited more potent activity than that of hexane and ethyl acetate extracts. No inhibitory effect was found against Pseudomonas aeruginosa check for this species in other resources , Salmonella typhi check for this species in other resources , and Klebsiella pneumoniae check for this species in other resources . Staphylococcus aureus check for this species in other resources required about 256 μg/ml of the crude methanol extract for effective inhibition. HPTLC evaluation at λ 254 nm was performed for the separation of a complex mixture of the methanol extract. The results provide evidence that Anabaena sp. extracts might indeed be potential sources of new antibacterial agents.

Keywords
Blue-green algae, drug-resistance, biologically active substances, bacterial species

 
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