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Indian Journal of Pharmacology
Medknow Publications on behalf of Indian Pharmacological Society
ISSN: 0253-7613
EISSN: 0253-7613
Vol. 43, No. 4, 2011, pp. 441-444
Bioline Code: ph11117
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Pharmacology, Vol. 43, No. 4, 2011, pp. 441-444

 en The protective effect of Rubia cordifolia check for this species in other resources against lead nitrate-induced immune response impairment and kidney oxidative damage
lodi, Shweta; Sharma, Veena & Kansal, Leena

Abstract

Objectives: To evaluate the in vivo antioxidant activity of the ethanolic extract of the roots of Rubia cordifolia check for this species in other resources (RC) and to study its influence on lead nitrate-induced impairment of immune responses.
Materials and Methods: Seventy-two adult male Swiss albino mice were used for biochemical and immunological studies and were divided into six groups of six mice each. Mice were treated with lead nitrate (40 mg/kg, orally) either alone and or in combination with RC (50 and 100 mg/kg body weight) daily for 40 days. For immunological studies, all mice were challenged twice with sheep RBC with on days 14 and 20 of the experiment. The immune function was assessed using macrophage yield, viability of macrophage, phagocytic index, serum immunoglobulin level, and plaque forming cell count (PFC), whereas the oxidative stress was assessed by estimating lipid peroxidation (LPO), reduced glutathione (GSH) content, and the activities of superoxide dismutase (SOD) and catalase (CAT).
Results: Lead nitrate administration induced a significant (P<0.001) increase in LPO, whereas a significant (P<0.001) depletion of CAT and GSH in renal tissues. In addition, it also showed a significant (P<0.001) reduction in macrophage yield, viability of macrophage, phagocyte index, serum immunoglobulin level, and PFC in kidney. However, combination treatment with RC observed a significant (P<0.001) reversal of lead nitrate-induced toxicity on oxidative stress and immunological parameters.
Conclusion: The lead nitrate-induced immunosuppression is due to oxidative stress and RC can prevent the same by virtue of its in vivo antioxidant property.

Keywords
Antioxidant, immunosuppression, lead nitrate, Rubia cordifolia

 
© Copyright 2011 Indian Journal of Pharmacology.
Alternative site location: http://www.ijp-online.com

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