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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 7, No. 2, 2008, pp. 953-959
Bioline Code: pr08013
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 7, No. 2, 2008, pp. 953-959

 en Uptake and kinetic properties of choline and ethanolamine in Plasmodium falciparum check for this species in other resources
Ahiboh, H.; Djaman, A.J.; Yapi, F.H.; Edjeme-Aké, A.; Hauhouot-Attoungbré, M.L.; Yayo, E.D. & Monnet, D.

Abstract

Purpose: The asexual proliferation of Plasmodium, inside the erythrocyte, is accompanied by the synthesis of huge quantities of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn). These needful phospholipids for the cytoplasmic membrane of the merozoites are provided by the precursors choline and ethanolamine. PtdCho and PtdEtn are synthesized by the parasite because the erythrocyte is unable to do it. In order to assess the dynamism of the phospholipid pathways, we aimed to investigate the respective shape of the uptake of choline and ethanolamine by Plasmodium falciparum check for this species in other resources
Method: Time-course experiments and kinetic assays were performed respectively with fixed and ranged concentrations of radioactively-labelled choline and ethanolamine. The labelled-precursors were added in the culture of P. falciparum infected-erythrocytes and the incorporated molecules in phospholipids were measured with a scintigraph counter.
Result: The results showed that the incorporation of precursors in the infected-erythrocyte occurred with a Michaelis-Menten’s kinetic shape. According to the maximum rate (V max ), the pathway of ethanolamine incorporation was faster than that of choline. Similarly, affinity for ethanolamine was greater than that of choline.
Conclusion: Although PtdCho is the major phospholipid in the membrane, this study rules out that the influx of ethanolamine in the infected-erythrocyte, in vivo conditions, is more dynamic than choline.

Keywords
Plasmodium, phospholipids, kinetics, metabolism, choline, ethanolamine

 
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