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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 7, No. 4, 2008, pp. 1151-1157
Bioline Code: pr08038
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 7, No. 4, 2008, pp. 1151-1157

 en Antimicrobial Activity of Flavonoids against Extended-Spectrum β-Lactamase (ESβL)-Producing Klebsiella pneumoniae check for this species in other resources
Özçelik, B; Orhan, D Deliorman; Özgen, S & Ergun, F

Abstract

Purpose: In the present study, six flavonoids (5,7-dimethoxyflavanone-4'-O-β-D-glucopyranoside, 5,7dimethoxyflavanone-4'-O-[2''-O-(5'''-O-trans-cinnamoyl)-β-D-apiofuranosyl]-β-D-glucopyranoside, naringenin-7-O-β-D-glucopyranoside, 5,7,3'-trihydroxy-flavanone-4'-O-β-D-glucopyranoside, rutin, and nicotiflorin) isolated from Galium fissurense check for this species in other resources , Viscum album ssp. album check for this species in other resources and Cirsium hypoleucum were screened against extended-spectrum β-lactamase producing multidrug-resistant (trimetoprimesulphametoxazole, sulbactam-ampicillin, clavulonate-amoxicilin, ceftriaxon, cefepime, imipenem, ceftazidime, tobramicin, gentamicin, ofloxacin, ciprofloxacin) bacteria Klebsiella pneumoniae check for this species in other resources (ESβLs).
Methods: We performed susceptibility testing according to the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) and used an inhibition endpoint for determination of the minimum inhibition concentrations (MICs).
Results: All the flavonoids showed in vitro antimicrobial activity against all the isolated strains of K. pneumoniae similar to the control antibacterial (ofloxacin) at the concentrations of 32 -64 µg ml-l; another control, ampicillin, had no activity. Since, ESβL-producing strains are known to be resistant to all β-lactam antibiotics, our results fall notably within the concentration range for antimicrobial activity.
Conclusion: To the best of our knowledge, this is the first report of the study of the activity of these flavonoids against (ESβL)-producing K. pneumoniae and may throw light to the low-toxicity of flavonoids, and their potentials for developing therapies for infections caused by ESβL-producing bacteria in the future. Further work is under investigation to identify their precise antibacterial mechanism.

Keywords
Antimicrobial activity, ESβLs, Flavonoids, Klebsiella pneumoniae, Ofloxacin, Ampicillin.

 
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