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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 8, No. 2, 2009, pp. 127-131
Bioline Code: pr09017
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 8, No. 2, 2009, pp. 127-131

 en In vitro Anti-Leishmanial and Anti-Tumour Activities of a Pentacyclic Triterpenoid Compound Isolated from the Fruits of Dregea volubilis Benth Asclepiadaceae check for this species in other resources
Moulisha, Biswas; Bikash, Mandal Nirup; Partha, Palit; Kumar, Ghosh Ashoke; Sukdeb, Bannerjee & Kanti, Haldar Pallab

Abstract

Purpose: Dregea volubilis Benth check for this species in other resources , commonly known as Jukti in Bengal, is used in the treatment of boils and abscesses from ancient times. The purpose of this study is to elucidate the active compounds and as well as their anti-leishmanial and anti-tumour activities.
Methods: Dried and crushed fruits of Dregea volubilis were extracted by petroleum ether (40 -60°C); the best solvent system had first been verified by analytical Thin Layer Chromatography (TLC). The extract was subjected to TLC and column chromatography (CC) to isolate the pure compounds. Spectra data were obtained by Infra Red pectroscopy, Mass Spectroscopy and Nuclear Magnetic Resonance -Proton Magnetic Resonance (PMR), Carbon Magnetic Resonance (CMR) and Distortionless Enhancement by Polarization Transfer (DEPT) -for structure elucidation of the isolated compound(s). One of the compounds isolated was screened for anti-leishmanial activity against promastigotes of Leishmania donovani and anti-tumour activity on K562 leukemic cell line.
Results: A pentacyclic triterpenoid compound was isolated and designated as taraxerone, and then characterized as d-friedoolean-14-en, 3 one together with ß-sitosterol and a long chain lipid fraction.. This compound showed in vitro anti-leishmanial activity against promastigotes of Leishmania donovani (strain AG 83) and anti-tumour activity on K562 leukemic cell line.
Conclusion: A pentacyclic triterpenoid compound designated as taraxerone and characterized as Dfriedoolean-14-en, 3 one together was successfully isolated. The structure was determined on the basis of spectral analysis (IR, MASS, NMR (PMR, CMR and DEPT) and the compound demonstrated in vitro anti-leishmanial and anti-tumour activities.

Keywords
Dregea volubilis, Pentacyclic triterpenoid, Taraxerone, Spectroscopy (IR, MASS, CMR, PMR, DEPT), Anti-leishmanial, Anti-tumour.

 
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