search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 8, No. 6, 2009, pp. 515-519
Bioline Code: pr09066
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 8, No. 6, 2009, pp. 515-519

 en Antidiarrhoeal Activity of Chromatographic Fractions of Stereospermum kunthianum check for this species in other resources Cham Sandrine Petit (Bignoniaceae) Stem Bark
Ching, F.P.; Okpo, S.O.; Falodun, A. & Omogbai, E.K.I.

Abstract

Purpose: The present study was undertaken in order to evaluate the antidiarrhoeal activity of three chromatographic fractions (L, S and Y) of Stereospermum kunthianum check for this species in other resources stem bark in mice.
Methods: Vacuum liquid/column chromatography (VLC/ CC) were used to obtain three fractions (L,S and Y) of Stereospermum kunthianum stem bark fractions. The antidiarrhoeal activities of these fractions at doses ranging from 100 – 400 mg/kg were evaluated in diarrhoea episodes induced by castor oil in mice. The controls were given distilled water (10 ml/kg) while 10 mg/kg of morphine was used as the reference drug (positive control).
Results: Pretreatment of mice with 100, 200 and 400 mg/kg of fraction L significantly (p < 0.05) delayed the onset of diarrhoea compared with the distilled water-treated mice. Fraction L at the dose of 400 mg/kg significantly reduced the number of wet stools in the treated mice. Fraction S at the doses of 200 and 400 mg/kg dose-dependently and significantly delayed the onset of diarrhoea in the treated mice. At the dose of 400 mg/kg, fraction S significantly reduced the number of wet stools and total number of stools as well as the total weight of wet and total weight of stools, compared to the distilled water-treated mice. Fraction Y at the three doses significantly delayed the onset of diarrhoea in the treated mice.
Conclusion: Vacuum liquid/column chromatography (VLC/CC) derived fractions L, S and Y of Stereospermum kunthianum possessed antidiarrhoeal activity but to varying degrees. This study lends further credence to the ethnomedicinal use of the plant for the treatment of diarrhoea.

Keywords
Antidiarrhoeal activity, chromatographic fractions, Stereospermum kunthianum, stools

 
© Copyright © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil