Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 8, No. 6, 2009, pp. 539-543
Bioline Code: pr09069
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 8, No. 6, 2009, pp. 539-543
© Copyright © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Development of Reverse-Phase HPLC Method for Simultaneous Analysis of Metoprolol Succinate and Hydrochlorothiazide in a Tablet Formulation|
Brijesh, Singh; Patel, D.K. & Ghosh, S.K.
To develop a simple, sensitive and rapid reverse phase HPLC method for the simultaneous
analysis of metoprolol succinate and hydrochlorothiazide in a solid dosage form.
The drugs were analysed by a reverse phase C-18 column using 50mM di-sodium hydrogen
phosphate:methanol:acetonitrile in a ratio of 525:225:250 as mobile phase. The flow rate was 1 ml/min
and the compounds were detected by a UV-detector at 222 nm at a column temperature of 24 ± 2 ºC.
The method was statistically validated for linearity and accuracy.
The retention time and drug content of metoprolol succinate and hydrochlorothiazide were 5.38
min, 96.05 % and 3.04 min., 97.64 %, respectively.
The study shows that the developed method is simple and accurate and that it would be
suitable for the simultaneous determination of metoprolol succinate and hydrochlorothiazide in
Metoprolol succinate; Hydrochlorothiazide; Simultaneous analysis; High performance liquid chromatography; Tablet formulation
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