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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 9, No. 2, 2010, pp. 135-140
Bioline Code: pr10016
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 9, No. 2, 2010, pp. 135-140

 en Anti-leishmanial and Anti-cancer Activities of a Pentacyclic Triterpenoid Isolated from the Leaves of Terminalia arjuna check for this species in other resources Combretaceae
Moulisha, Biswas; Kumar, Ghosh Ashok & Kanti, Haldar Pallab

Abstract

Purpose: Terminalia arjuna check for this species in other resources Roxb (Combretaceae) is commonly known as Arjjhan and Arjun in Bengal, India. The anti-leishmanial and anticancer activities of a pentacyclic triterpenoid isolated from its leaves were evaluated.

Methods: Dried and crushed leaves of Terminalia arjuna were de-fatted with petroleum ether (40 - 60°C) and extracted with methanol. The extract was subjected to column chromatography and column fractions were monitored on TLC plates developed in a suitable solvent system. Structures of the isolated pure compounds were elucidated by infra-red spectroscopy, mass spectrometry and nuclear magnetic resonance spectroscopy. One of the compounds was screened for anti-leishmanial activity against promastigotes of Leishmania donovani check for this species in other resources , and anti-cancer activity on K562 leukaemic cell line.

Results: A pentacyclic triterpenoid, characterized as 3ß-hydroxyurs-12-en-28-oic acid (together with ß- D-glucoside) and designated ursolic acid, was successfully isolated. The structure was determined on the basis of spectroscopic analyses. In vitro anti-leishmanial activity against promastigotes of Leishmania donovani (strain AG 83) and anti-cancer activity on K562 leukaemic cell line were shown by the isolated ursolic acid.

Conclusion: The methanol extract of the leaves of Terminalia arjuna led to the isolation of a pentacyclic triterpenoid, ursolic acid. This compound demonstrated in vitro anti-leishmanial and anti-cancer activities.

Keywords
Terminalia arjuna Roxb, Pentacyclic triterpenoid, Ursolic acid, Spectroscopy, Antileishmanial, Anti-cancer

 
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