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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 9, No. 2, 2010, pp. 149-156
Bioline Code: pr10018
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 9, No. 2, 2010, pp. 149-156

 en Effect of Ethanol Extract of Indigofera tinctoria check for this species in other resources Linn (Fabaceae) on Lithium/Pilocarpine-Induced Status Epilepticus and Oxidative Stress in Wistar Rats
Asuntha, G.; Prasannaraju, Y. & Prasad, K.V.S.R.G.

Abstract

Purpose: Indigofera tinctoria check for this species in other resources Linn. of the Fabaceae family is claimed to be useful in the treatment of a variety of epileptic disorders in Indian traditional system of medicine. The present study was designed to verify this claim.

Methods: Status epilepticus was induced in male albino rats of Wistar strain by administration of pilocarpine (30 mg/kg, i.p.) 24 h after injection of lithium chloride (3 mEq/kg, i.p.). Different doses of the ethanol extract of Indigofera tinctoria were administered orally 1 h before the injection of pilocarpine. The severity of status epilepticus was observed and recorded every 15 min for 90 min and thereafter every 30 min for another 90 min, using Racine scoring system. In-vivo lipid peroxidation of rat brain tissue was measured in terms of the thiobarbiturate-reactive substances (TBARS). Both in-vitro free radical nitric oxide (NO) and 2-diphenyl-2-picryl hydrazyl (DPPH) scavenging activities of the extract were also determined.

Results: The severity of status epilepticus was significantly (p < 0.01) reduced following oral administration of the extract at 500 and 1000 mg/kg doses. No test animal group exhibited stage 4 seizure. The extract also exhibited both in-vivo and in-vitro antioxidant activities.

Conclusion: The ethanol extract of Indigofera tinctoria was found to be useful in controlling lithium/pilocarpine-induced status epilepticus in albino rats.

Keywords
Indigofera tinctoria, Free radical scavenging, Status epilepticus, Antioxidant, Albino rats, Polyphenols

 
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