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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 9, No. 3, 2010, pp. 231-236
Bioline Code: pr10028
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 9, No. 3, 2010, pp. 231-236

 en Isolation and morphological characterization of antibiotic producing actinomycetes
Nanjwade, Basavaraj K.; Chandrashekhara, S.; Shamarez, Ali M.; Goudanavar, Prakash S. & Manvi, Fakirappa V.

Abstract

Purpose: To isolate and characterize antibiotic producing actinomycetes from soil samples in Belgaum, Karnataka, India.
Methods: Crowded plate technique was used for the isolation of actinomycetes in media such as soybean - casein digest medium and actinomycetes isolation agar. The morphological and cultural characterization of one of the selected strains, designated A-4, was performed as per International Streptomycete Project (ISP).
Results: Morphological and cultural studies showed that A-4 belonged to the Actinomycete genus. The morphological and cultural characteristics of the A-4 mutant showed cellular and aerial growth as well as soluble pigment formation in various ISP media.
Conclusion: Findings from this investigation revealed that the selected strain, A-4, is an actinomycete.

Keywords
Actinomycete, Antibiotic, MIC, Crowded plate technique, Morphology

 
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