Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 9, No. 5, 2010, pp. 455-462
Bioline Code: pr10054
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 9, No. 5, 2010, pp. 455-462
© Copyright 2010 Tropical Journal of Pharmaceutical Research.
In Vitro Antitumour Activity of Sesamum indicum Linn Flower Extracts|
Xu, Hua; Wen, Yong-ping; Zhao, Wen & He, Qing
Purpose: To investigate the antitumour activity of Sesamum indicum L.
Methods All the fractions of the ethanol extract of Sesamum indicum L. were obtained by solvent
refining technique. Methyl thiazolyl tetrazolium (MTT) method was used to test the cytotoxicity of the
extracts on four different tumour cell lines - Hela, CNE-2, smmc-7721 and Kyse-410. The effect of
Sesamum indicum L. on apoptosis and cell cycle of tumour cells was analyzed by flow cytometry.
Results The petroleum benzene fraction and diethyl ether fraction showed significant antitumour activity
compared with the control (P<0.05). Typical hypodiploid apoptotic peak was demonstrated in tumour
cells treated by petroleum benzene fraction. The diethyl ether fraction evidently influenced the cell cycle
of the tumour cells.
Conclusion The petroleum benzene and diethyl ether fractions of Sesamum indicum L. may contain
antitumour components. The mechanism of inhibition of tumour cells may be linked to cytotoxic activity
and apoptotic induction.
Sesamum indicum L.; Anti-tumour activity; MTT method; Flow cytometry.
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