Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 9, No. 5, 2010, pp. 463-467
Bioline Code: pr10055
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 9, No. 5, 2010, pp. 463-467
© Copyright 2010 Tropical Journal of Pharmaceutical Research.
Acute and Sub-chronic (28-day) Oral Toxicity Studies of Hydroalcohol Leaf Extract of Ageratum conyzoides L (Asteraceae)|
Diallo, Aboudoulatif; Eklu-Gadegkeku, Kwashie; Agbonon, Amegnona; Aklikokou, Kodjo; Creppy, Edmond E. & Gbeassor, Messanvi
Purpose: Ageratum conyzoides is an annual herbaceous plant commonly used in African traditional
medicine as a purgative, antipyretic, anti-ulcer and wound dressing agent. The objective of this study
was to investigate the acute and sub-chronic toxicity of A. conyzoides leaves in Wistar rats.
Methods: In the acute test, the limit test dose of 5000 mg/kg was administered to Wistar rats and then
observed individually 1 h post-dosing, and at least once daily for 14 days. Sub-chronic toxicity was
evaluated after administering daily oral doses of 500 and 1000 mg/kg body wt., for 28 days to the rats,
Biochemical and haematological assessments as well as body and relative organ weights of the rats
were carried out
Results: The limit dose of 5000 mg/kg did not cause any mortality or signs of acute toxicity in the rats
tested during the observation period. In the sub-chronic tests, the results did not show any treatment–
related abnormalities in terms of haematological and biochemical parameters. However, urea was
significantly (p < 0.05) lower in the group treated with 500 mg/kg of A. conyzoides extract. The weekly
body and organ weight of the rats showed no significant differences between the control and the rats
treated with the extract except for liver where there was a significant increase (p < 0.05) in rats that
received 1000 mg/kg, i.e., 3 ± 0.2 g as against 2.5 ± 0.1 g for the control.
Conclusion: Our results suggest that the hydroalcohol extract of A. conyzoides is relatively safe when
administered orally in rats.
Ageratum conyzoides, Acute and sub-chronic toxicity, Biochemical parameters, Haematological analysis, Wistar rats.
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