Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 9, No. 5, 2010, pp. 469-473
Bioline Code: pr10056
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 9, No. 5, 2010, pp. 469-473
© Copyright 2010 Tropical Journal of Pharmaceutical Research.
In Vitro Anticholinesterase and Inhibitory Effects of the Aqueous Extract of Combretum molle (Combretaceae) Leaf on Rabbit Breathing|
Dodehe, Yéo; Ernest, Koffi; Philippe, Bidié Alain dit; Antoine, Tako Némé; Calixte, Bahi; Souleymane, Méité; Joseph, Djaman Allico & Frédéric, Guéde-Guina
Purpose: In previous studies, the aqueous extract of Combretum molle was shown to inhibit
disturbances of breathing induced by acetylcholine. The aim of this study was to elucidate the
mechanism of this inhibition.
Methods: The aqueous extract of Combretum molle and an anti-asthmatic, salbutamol
(reference), were tested at varying concentrations for their inhibitory effects on breathing.
Acetylcholinesterase (AChE) was extracted from rabbit duodenum and its activity determined
by Ellman’s assay using acethylthiocholine iodide (ACTH) as substrate. The rate of hydrolysis
of acetylcholinesterase was spectrophotometrically monitored at 412 nm.
Results: The results indicate that in the presence of C. molle extract, a considerable
reduction in the variation of breathing amplitudes occurred. Furthermore, the extract probably
contains anti-AChE substances. C. molle exerted non-competitive inhibition of
acetylcholinesterase with Michaelis-Menten constant (KM) of 192 μM and velocity at maximal
concentration of substrate (Vmax) of 4444 μM/min.
Conclusion: These results support the use of C. molle leaf in the Pharmacopoeia of Ivory
Coast as an anti-asthmatic, in view of its inhibitory effect on breathing disturbances.
Combretum molle, Acetylcholinesterase, Breathing disturbances, Salbutamol, Anti-asthmatic
Alternative site location: http://www.tjpr.org