About Bioline  All Journals  Testimonials  Membership  News  Donations

Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 9, No. 6, 2010, pp. 587-593
Bioline Code: pr10071
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 9, No. 6, 2010, pp. 587-593

 en Reverse Phase High Pressure Liquid Chromatographic Determination of Rifampin Quinone and Hydrazone in Anti-tuberculosis Fixed-Dose Formulations Containing Sodium Ascorbate as Anti-oxidant
Umapathi, P.; Ayyappan, J. & Quine, Darlin


Purpose: To establish the method for the analysis of rifampin quinone and hydrazone in fixeddose combination tablets (FDC) containing rifampicin with or without sodium ascorbate as an additive.
Methods: The International Pharmacopoeial (IP) method for the estimation of rifampin quinone in FDC was modified. The extraction solvent (methanol/buffer mixture) of the IP method was replaced with ethyl acetate, in order to stabilize rifampin quinone in sample solutions of FDC containing sodium ascorbate as an ingredient.
Results: Rifampin quinone in FDC samples containing sodium ascorbate, which is not detectable in the IP method, was found to be 1.15 % (4-FDC), 1.52 % (3-FDC) and 1.60 % (2-FDC) using the modified method. Recovery of rifampin quinone in spiked samples of FDCs was practically nil in IP method whereas the recovery was 99.95%, 99.39 and 99.02 % for 4–FDC, 3-FDC and 2-FDC, respectively.
Conclusion: This modified method was suitable for the determination of rifampin quinone in fixed-dose formulations of rifampin both in the presence and absence of sodium ascorbate. The method is specific, precise, accurate, robust, rugged and gives a linear response for the quantitative estimation of rifampin quinone and hydrazone in fixed-dose combination tablets containing rifampin.

Fixed dose combination; Rifampin quinone; Tuberculosis; Sodium ascorbate; Quantitative determination; HPL

© Copyright 2010 Tropical Journal of Pharmaceutical Research.
Alternative site location:

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil